{"in": ["the strains"], "out": "pellet"}
{"in": ["pellet"], "out": "cells"}
{"in": ["cells"], "out": ""}
{"in": ["ethanol dry ice bath"], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": "the top layer (aqueous phase)"}
{"in": ["phenol extraction", "phenol:chloroform (pH 4.7)"], "out": "the RNA"}
{"in": ["cold 100% ethanol", "the RNA"], "out": "the RNA pellet"}
{"in": ["cold sodium acetate (0.3 M, pH 4.5)", "the RNA pellet", "the top layer (aqueous phase)"], "out": ""}
{"in": [], "out": ""}
{"in": [], "out": "the pellet"}
{"in": ["the pellet"], "out": "the RNA"}
{"in": ["the RNA"], "out": "spectrophotometry (A260)"}