{"action": "centrifuge", "output": "", "temperature": ["<<<room temperature>>>"], "time": ["5 min"], "force": ["<<<200 x g>>>"]}
{"action": "remove", "output": "the supernatant", "reagent": [""]}
{"action": "suspend", "output": "", "volume": ["2 ml"], "reagent": ["ACK lysing buffer"], "time": ["1 min"]}
{"action": "repeat", "output": "", "reagent": ["the ACK"]}
{"action": "rinse", "output": "", "container": ["the cell suspension", "a 40 \u03bcm nylon strainer"], "device": [""], "reagent": ["the supernatant"]}
{"action": "wash", "output": "", "container": ["the strainer"], "reagent": ["<<<2 ml 1x DPBS>>>"], "time": ["5 min"]}
{"action": "wash", "output": "", "reagent": ["1x DPBS with 20 ng/ml murine M-CSF"], "container": ["a 100 mm Petri dish"]}
{"action": "suspend", "output": "", "volume": ["<<<15 ml>>>"], "reagent": ["complete DMEM medium"]}
{"action": "incubate", "output": "fresh complete DMEM medium", "temperature": ["<<<37 \u00b0C>>>"]}
{"action": "replace", "output": "", "reagent": ["fresh complete DMEM medium"]}
{"action": "repeat", "output": "", "reagent": ["fresh complete DMEM medium"]}