{"action": "grow", "output": "", "reagent": ["YPD media"], "length": [""], "device": [""]}
{"action": "harvest", "output": "sterile water", "container": ["50 ml tubes"], "device": ["centrifugation"], "speed": ["<<<1,000 x g>>>"], "time": ["<<<5 min>>>"]}
{"action": "wash", "output": "", "reagent": ["sterile water"], "temperature": ["<<<room temperature>>>"], "time": [""]}
{"action": "resuspend", "output": "", "volume": ["50 ml culture"]}
{"action": "incubate", "output": "DTT", "time": ["30 min"], "temperature": ["<<<30 \u00b0C>>>"]}
{"action": "add", "output": "", "reagent": ["DTT"]}
{"action": "incubate", "output": "", "container": ["the suspension(s)"], "temperature": ["30 \u00b0"], "reagent": [""], "time": ["<<<60 min>>>"]}
{"action": "add", "output": "", "volume": ["40 ml"], "reagent": ["sterile pre-cooled water"]}
{"action": "centrifuge", "output": "", "container": ["the suspensions"], "force": [""], "time": [""]}
{"action": "resuspend", "output": "", "container": ["cell pellets"], "reagent": ["<<<sterile water>>>"], "volume": ["<<<25 ml>>>"], "temperature": ["4 \u00b0C"]}
{"action": "repeat", "output": "", "reagent": [""]}
{"action": "resuspend", "output": "", "container": ["<<<cell pellets>>>"], "volume": ["5 ml"], "reagent": [""]}
{"action": "repeat", "output": "", "reagent": [""]}
{"action": "resuspend", "output": "", "concentration": ["pre-cooled 1 M sorbitol"], "volume": ["<<<550 \u00b5l>>>"]}
{"action": "add", "output": "", "volume": ["5 to 10 \u00b5l"], "reagent": ["highly purified transforming DNA (1 to 3 \u00b5g)"]}
{"action": "incubate", "output": "YPD media", "temperature": ["ice"], "time": ["<<<10 min>>>"]}
{"action": "add", "output": "", "reagent": ["YPD media"], "container": ["each cuvette"]}
{"action": "transfer", "output": "", "container": ["the suspension"]}
{"action": "add", "output": "", "volume": ["45 \u00b5l"], "reagent": ["electrocompetent cell suspensions"]}
{"action": "incubate", "output": "", "container": ["the plate"], "temperature": ["ice"], "time": ["10 min"]}
{"action": "make", "output": "", "length": [""]}
{"action": "cover", "output": "YPD media", "container": ["the plate"], "device": ["a sterile lid"]}
{"action": "transfer", "output": "", "container": ["the cell suspension"], "reagent": ["YPD media"]}
{"action": "use", "output": "", "volume": [""]}
{"action": "centrifuge", "output": "sterile water", "container": ["the tubes"], "force": ["1,000 x g"], "time": ["5 min"]}
{"action": "remove", "output": "", "reagent": ["sterile water"]}