{"action": "centrifuge", "output": "", "speed": ["17,500 \u00d7 g"], "time": ["1 h"], "temperature": ["4 \u00b0C"]}
{"action": "remove", "output": "the pellets", "reagent": [""]}
{"action": "divide", "output": "the supernatants (soluble fractions)", "volume": ["<<<10 \u00b5L>>>"], "temperature": ["<<<-80 \u00b0C>>>"]}
{"action": "perform", "output": "the BCA protein assay kit", "device": ["SDS-PAGE"]}
{"action": "measure", "output": "", "concentration": ["both positive cell lysates"], "reagent": ["the BCA protein assay kit", "the supernatants (soluble fractions)"]}
{"action": "rinse", "output": "all future DNA library", "reagent": ["<<<100 mM HCl>>>"]}
{"action": "prepare", "output": "", "volume": ["10 \u00b5L"], "reagent": ["naive DNA library"]}
{"action": "use", "output": "", "reagent": ["this treatment", "all future DNA library"]}
{"action": "prepare", "output": "", "concentration": ["100 \u00b5M"], "reagent": ["temperature-treated DNA library (from step 6)"]}
{"action": "prepare", "output": "DNA", "concentration": ["10 \u00b5M DNA library"]}
{"action": "amplify", "output": "", "reagent": ["<<<DNA>>>"]}
{"action": "incubate", "output": "the DNA library", "temperature": ["<<<room temperature>>>"], "time": ["<<<10 minutes>>>"]}
{"action": "determine", "output": "the free DNA", "reagent": ["the DNA library"]}
{"action": "perform", "output": ""}
{"action": "determine", "output": "", "reagent": ["fluorescein", "the free DNA"]}