{"action": "add", "output": "", "reagent": ["exo-resistant"], "volume": ["1 \u00b5l"]}
{"action": "keep", "output": "\u00b5l stop buffer (400 mM HCl, 600 mM Tris-HCl pH 7.5)", "temperature": ["ice"], "time": ["<<<10 min>>>"], "reagent": [""]}
{"action": "add", "output": "", "reagent": ["\u00b5l stop buffer (400 mM HCl, 600 mM Tris-HCl pH 7.5)"], "volume": ["3 \u00b5l"]}
{"action": "keep", "output": "2 \u00b5l Phi29 polymerase", "temperature": ["<<<ice>>>"], "time": ["<<<2 min>>>"], "reagent": [""]}
{"action": "combine", "output": "", "volume": ["32 \u00b5l master mix 30 \u00b5l MDA reaction buffer,"], "reagent": ["2 \u00b5l Phi29 polymerase"]}
{"action": "remove", "output": "clear liquid", "reagent": [""]}
{"action": "rinse", "output": "", "reagent": ["<<<beads>>>"]}
{"action": "minimize", "output": "nuclease-free water", "length": [""]}
{"action": "elute", "output": "", "reagent": ["<<<nuclease-free water>>>"], "volume": ["32 \u00b5l"]}
{"action": "use", "output": "", "device": ["<<<magnetic separator>>>"]}
{"action": "transfer", "output": "", "volume": ["30 \u00b5l"], "container": ["tube"], "reagent": ["clear liquid"]}
{"action": "quantify", "output": "TBE,", "mass": ["<<<10-12 \u00b5g>>>"], "reagent": ["", ""]}
{"action": "check", "output": "product size", "device": ["agarose gel electrophoresis"]}
{"action": "prepare", "output": "", "container": ["<<<96-well plate>>>"], "reagent": ["PCR mixture", "product size"]}
{"action": "incubate", "output": "", "device": ["<<<PCR machine>>>"], "temperature": ["<<<95\u00b0C>>>"], "time": ["<<<20s>>>"]}
{"action": "calculate", "output": "relative uniformity values (RUVs)"}