{"action": "cut", "device": ["a cryostat"], "output": ""}
{"action": "display", "output": "", "string": [""], "time": [""]}
{"action": "rotate", "output": "", "temperature": ["-20\u00baC"]}
{"action": "remove", "output": "the blocking solution", "reagent": [""]}
{"action": "prepare", "output": "", "reagent": ["the blocking solution"]}
{"action": "incubate", "output": "1:5,000 rabbit anti-GFP (abcam ab290) -NeuN (Millipore ABN90P)", "reagent": ["the blocking solution"], "temperature": [""], "time": ["1 hour"]}
{"action": "make", "output": "", "reagent": ["1:5,000 rabbit anti-GFP (abcam ab290) -NeuN (Millipore ABN90P)"]}
{"action": "perform", "frequency": ["three times"], "output": "anti-mouse AlexaFluor647", "reagent": [""]}
{"action": "include", "output": "synaptic co-localization", "reagent": ["anti-mouse AlexaFluor647"]}
{"action": "add", "output": "", "reagent": ["hoechest (1:1,000)"]}