{"action": "grow", "output": "pellet", "reagent": ["the strains"]}
{"action": "suspend", "output": "cells", "reagent": ["pellet"]}
{"action": "transfer", "container": ["glass beads"], "output": "", "reagent": ["cells"], "temperature": ["\u201320 \u00b0C"]}
{"action": "grab", "output": "", "reagent": ["ethanol dry ice bath"]}
{"action": "hold", "device": [""], "output": ""}
{"action": "add", "container": ["the samples"], "output": "", "volume": ["300 mL phenol:chloroform (pH 4.7)"]}
{"action": "vortex", "device": ["eppendorf tubes"], "output": "", "temperature": ["ice"]}
{"action": "transfer", "container": ["new tubes"], "output": "the top layer (aqueous phase)"}
{"action": "repeat", "output": "the RNA", "reagent": ["phenol extraction", "phenol:chloroform (pH 4.7)"], "temperature": ["4 \u00b0C"], "time": ["15 minutes"]}
{"action": "precipitate", "output": "the RNA pellet", "reagent": ["cold 100% ethanol", "the RNA"], "temperature": ["4 \u00b0C"], "time": ["25 minutes"]}
{"action": "suspend", "concentration": [""], "output": "", "reagent": ["cold sodium acetate (0.3 M, pH 4.5)", "the RNA pellet", "the top layer (aqueous phase)"], "volume": ["60 \u00b5L"]}
{"action": "precipitate", "output": "", "temperature": ["4 \u00b0C"], "volume": ["400 \u00b5L 100% ethanol"]}
{"action": "dry", "device": [""], "output": "the pellet", "temperature": ["ice"]}
{"action": "resuspend", "container": ["the pellet"], "output": "the RNA", "reagent": ["the pellet"], "volume": ["50 \u00b5L"]}
{"action": "evaluate", "output": "spectrophotometry (A260)", "reagent": ["the RNA"]}