{"action": "prepare", "output": "recipes 1", "reagent": ["reagents"]}
{"action": "note", "device": [""], "output": "", "reagent": ["DEPC water"]}
{"action": "collect", "device": ["centrifuge"], "output": "", "reagent": ["cells"]}
{"action": "spin", "output": "AE buffer", "reagent": ["cells"], "time": ["15 sec"], "volume": ["1 mL water"]}
{"action": "suspend", "output": "", "reagent": ["AE buffer"], "temperature": ["room temperature"], "volume": ["400 \u00b5L"]}
{"action": "add", "output": "", "reagent": ["10% SDS"], "volume": ["40 \u00b5L"]}
{"action": "add", "output": "", "reagent": ["hot phenol/AE"], "temperature": ["65 \u00b0C"], "volume": ["500 \u00b5L"]}
{"action": "sort", "output": "", "reagent": ["phenol/AE"], "time": ["1 min"]}
{"action": "incubate", "output": "", "temperature": ["65 \u00b0C"], "time": ["5 min"]}
{"action": "suspend", "output": "", "reagent": ["liquid nitrogen"]}
{"action": "see", "output": "", "reagent": ["cells"], "temperature": ["30 \u00b0C"]}
{"action": "transfer", "output": "supernatant", "volume": ["400 \u03bcL"]}
{"action": "add", "output": "", "reagent": ["phenol: CHCl3/AE-Na"], "volume": ["400 \u00b5L"]}
{"action": "spin", "device": ["a standard laboratory microfuge"], "force": ["20,000 x g"], "output": "CHCl3", "reagent": ["supernatant"], "time": ["5 min"]}
{"action": "add", "container": ["isoamyl alcohol (24:1)"], "output": "", "reagent": ["CHCl3"]}
{"action": "transfer", "container": ["a fresh 1.5 mL microfuge tube"], "output": "aqueous supernatant"}
{"action": "repeat", "container": ["the aqueous phase"], "output": "", "reagent": ["aqueous supernatant"]}
{"action": "add", "output": "", "reagent": ["3 M NaOAc (pH 5)"], "volume": ["1/10 volume"]}
{"action": "add", "output": "", "volume": ["ethanol"]}
{"action": "place", "output": "", "temperature": ["-20 \u00b0C"], "time": ["at least 30 min"]}
{"action": "spin", "device": ["the microfuge"], "force": ["20,000 x g"], "output": "", "temperature": ["4 \u00b0C"], "time": ["15 min"]}
{"action": "add", "output": "the liquid droplets", "reagent": ["ice-cold 75% EtOH"], "temperature": ["4 \u00b0C"], "time": ["around 10 min"]}
{"action": "pour", "container": ["the tube"], "output": "", "reagent": ["the liquid droplets"]}
{"action": "add", "container": ["\u00b5L ddH2O (DEPC)"], "output": "RNA", "volume": ["30-50 \u00b5L"]}
{"action": "dissolve", "output": "", "reagent": ["RNA"], "temperature": ["37 \u00b0C"], "time": ["30 min"]}