{"action": "determine", "device": ["Casy cell counter"], "output": "cell count"}
{"action": "mix", "mass": ["0.5 \u00b5g HyPerER"], "output": "Fugene\u00ae HD solution", "volume": ["25 \u00b5l"]}
{"action": "incubate", "output": "", "reagent": ["Fugene\u00ae HD solution"], "temperature": ["room temperature"], "time": ["15 min"]}
{"action": "add", "container": ["cells"], "output": "cells", "reagent": ["cell count"]}
{"action": "add", "output": "", "reagent": ["growth medium"], "volume": ["2.6 ml"]}
{"action": "incubate", "output": "", "reagent": ["cells"], "temperature": ["37\u00b0C"], "time": ["24-48 h"]}
{"action": "perform", "device": ["Olympus Fluoview 3000 microscope"], "output": "culture medium"}
{"action": "remove", "output": "pre-warmed HEPES Imaging Buffer", "reagent": ["culture medium"]}
{"action": "rinse", "output": "", "reagent": ["pre-warmed HEPES Imaging Buffer"], "volume": ["1 ml"]}
{"action": "add", "device": ["microscope stage"], "output": "stimulating substances (e.g., thapsigargin)", "reagent": [""], "volume": ["1 ml HEPES Imaging Buffer"]}
{"action": "add", "output": "baseline oxidative status", "reagent": ["stimulating substances (e.g., thapsigargin)"]}
{"action": "proceed", "output": "measurement", "reagent": ["100 \u00b5M H2O2"]}