{"action": "prepare", "concentration": ["50 \u00b5M RNA/DNA oligos"], "container": ["PCR tube"], "output": "inhibitor compound", "reagent": ["DNase/[RNase-free] water"], "volume": ["50 \u00b5l"]}
{"action": "dissolve", "output": "water", "reagent": ["inhibitor compound"]}
{"action": "add", "container": ["96-well plate"], "output": "", "reagent": ["water"]}
{"action": "add", "output": "", "volume": ["80 \u00b5l RT reaction mix (1.25x)"]}
{"action": "add", "container": ["samples"], "output": "EDTA", "volume": ["10 \u00b5l inhibitor dilution"]}
{"action": "stop", "output": "", "reagent": ["EDTA"], "volume": ["50 \u00b5l"]}
{"action": "quantify", "device": ["Victor 3"], "output": "490/528 nm"}
{"action": "subtract", "output": "blank value", "reagent": ["490/528 nm"]}
{"action": "calculate", "concentration": ["IC50 value"], "output": "signal"}