{"action": "synthesize", "output": "nanoparticles", "reagent": [""]}
{"action": "cool", "output": "", "reagent": ["ethanol"], "temperature": ["room temperature"]}
{"action": "remove", "output": "the brown supernatant", "reagent": ["nanoparticles"]}
{"action": "rinse", "output": "leftover nonmagnetic product", "reagent": ["ethanol"]}
{"action": "note", "output": "hydrophilic reaction step", "reagent": ["ethanol"]}
{"action": "add", "output": "black powders", "reagent": ["water"]}
{"action": "adjust", "output": "TGA", "time": [""]}
{"action": "determine", "output": "FIASH-EDT2", "reagent": ["TGA"]}
{"action": "perform", "output": "", "reagent": ["FIASH-EDT2", "Maleimide", "SPMNPs"]}
{"action": "perform", "output": "the reaction", "temperature": ["room temperature"]}
{"action": "remove", "device": ["a magnetic field"], "output": "PBS", "reagent": ["unbound (fluorescein)"]}
{"action": "rinse", "output": "further cellular uptake analysis", "reagent": ["PBS"]}
{"action": "incubate", "output": "", "reagent": ["fluorescence-modified SPMNPs"], "temperature": ["37\u00b0C"], "time": ["different Pulse\u2013Chase periods"]}
{"action": "fix", "output": "DAPI nucleus staining", "reagent": ["4% paraformaldehyde"]}
{"action": "collect", "device": ["an upright Nikon Eclipse E800 microscope"], "output": ""}
{"action": "process", "output": ""}