{"action": "obtain", "output": "fresh blood specimens"}
{"action": "identify", "output": "MACS buffer", "reagent": ["Lymphoprep density gradient medium", "PBMC"]}
{"action": "isolate", "device": ["CD4<sup>+</sup> T cell Isolation Kit"], "output": "", "reagent": ["total CD4<sup>+</sup> T cells"]}
{"action": "prepare", "container": ["LS column"], "output": "", "reagent": ["MACS buffer"], "volume": ["3 ml"]}
{"action": "wash", "output": "", "reagent": ["CD4<sup>+</sup> T cells (negative fraction)"]}
{"action": "treat", "output": "", "reagent": ["Treg cells"], "time": ["24 hs"]}
{"action": "leave", "concentration": ["0.5-1.0\u00d710^5 cells per well"], "output": ""}
{"action": "rinse", "output": "the resulting Treg cells", "reagent": ["culture medium"]}
{"action": "estimate", "concentration": [""], "container": ["the cells"], "output": "CFSE", "reagent": ["the resulting Treg cells"], "volume": [""]}
{"action": "rinse", "output": "", "reagent": ["CFSE"]}
{"action": "maintain", "concentration": ["2\u00d710<[sup>3</sup>] anti-CD3/CD28 beads"], "output": "", "temperature": [""], "time": ["4 days"]}
{"action": "maintain", "concentration": ["2\u00d710<[sup>3</sup>] anti-CD3/CD28 beads"], "output": "", "temperature": [""], "time": ["4 days"]}
{"action": "connect", "device": [""], "output": "percent inhibition"}