{"action": "cut", "output": ""}
{"action": "remove", "container": ["the mesentery"], "device": [""], "output": "PBS", "reagent": [""]}
{"action": "rinse", "output": "", "reagent": ["PBS"]}
{"action": "cut", "device": ["a scissor"], "output": ""}
{"action": "cut", "device": ["Leica CM3050 cryostat"], "length": ["5 \u03bcm"], "output": ""}
{"action": "fix", "output": "", "temperature": ["ice-cold acetone"], "time": ["seven minutes"]}
{"action": "store", "container": ["desiccant bags"], "output": "", "temperature": ["-80\u00b0C"]}
{"action": "handle", "device": ["a Dako Pen"], "output": "staining", "reagent": [""]}
{"action": "block", "output": "TBS-T", "reagent": ["TBS-T", "antibodies"]}
{"action": "dip", "output": "", "reagent": ["TBS-T"]}
{"action": "apply", "output": "the staining solutions", "reagent": ["TBS-T"], "time": ["45 minutes"]}
{"action": "rinse", "output": "DAPI nucleic acid stain", "reagent": ["the staining solutions"]}
{"action": "stain", "output": "PBS", "reagent": ["DAPI nucleic acid stain"], "time": ["30 seconds"]}
{"action": "rinse", "device": ["slide-beakers"], "output": "", "reagent": ["PBS"]}
{"action": "acquire", "device": ["Leica DMRA2 fluorescence microscope"], "output": ""}
{"action": "keep", "output": ""}
{"action": "apply", "device": [""], "output": "images"}
{"action": "estimate", "device": ["ImageJ"], "output": "small intestinal LP IgA+ plasma cells"}
{"action": "quantify", "output": "five separate images", "reagent": ["images"]}