Make a midline incision (20–30 mm) to open the abdomen.
{"action": "make", "output": "", "container": ["the abdomen"]}
mount the ileocecal portion of the mesentery on a transparent plastic stage
{"action": "handle", "output": "", "container": ["the mesentery"], "reagent": [""]}
Maintain the mesentery at 37 ºC with continuous saline superfusion. #####%%%%% output "continuous saline superfusion" are not used
{"action": "maintain", "output": "continuous saline superfusion", "temperature": ["37 \u00baC"]}
If the selected venule shows leukocyte adhesion , repeat the selection process. #####%%%%% output "leukocyte adhesion" are not used
{"action": "repeat", "output": "leukocyte adhesion", "reagent": [""]}
Ensure no leukocyte adhesion .
{"action": "ensure", "output": "", "reagent": ["no leukocyte adhesion"], "time": [""]}
Induce ischemia by ligating the anterior mesenteric artery for 10 min.
{"action": "induce", "output": "", "time": ["10 min"], "reagent": ["ischemia"], "device": ["ligating the anterior mesenteric artery"]}
Reperfusion: cut the snare to restore blood flow. #####%%%%% output "blood flow" are used in line16
{"action": "cut", "output": "blood flow", "device": ["the snare"]}
If reperfusion is not successful, repeat the ligation steps. #####%%%%% output "the ligation steps" are not used
{"action": "repeat", "output": "the ligation steps", "reagent": ["blood flow"]}
Observe microcirculation using LOSOM to track leukocyte rolling .
{"action": "observe", "output": "", "device": ["LOSOM"], "reagent": ["microcirculation"]}