Drain off excess mineral oil with the gel facing upwards.
{"action": "drain", "output": "", "reagent": ["mineral oil"]}
For each IPG DryStrip, cut two electrode wicks 2 cm in length.
{"action": "cut", "output": "", "device": ["two electrode wicks"], "length": ["2 cm"]}
Soak electrode wicks with 100 µL HPLC grade water with a clean wipe to ensure that the wicks are damp .
{"action": "soak", "output": "", "device": ["electrode wicks"], "volume": ["100 µL"], "reagent": ["HPLC grade water"]}
Place damp wicks on either end of the IPG strips .
{"action": "place", "output": "", "device": ["damp wicks"]}
fix electrode bars onto the wicks at either end of the IPG strips #####%%%%% output "mineral oil" are used in line12
{"action": "fix", "output": "mineral oil", "container": ["the wicks"]}
Pour mineral oil into the Multiphor sample tray until the strips are immersed.
{"action": "pour", "output": "", "reagent": ["mineral oil"], "container": ["the Multiphor sample tray"]}
Prod wicks gently using tweezers to remove air bubbles . #####%%%%% output "air bubbles" are not used
{"action": "prod", "output": "air bubbles", "device": ["tweezers"]}
Carry out IEF at 300 V for 2 h, a gradient increase to 3500 V over 3 h, .
{"action": "carry", "output": "", "device": ["IEF"], "time": ["2 h"]}
then maintain at 3500 V up to 70 kVh using an EPS 3500XL power supply
{"action": "maintain", "output": "", "device": ["EPS 3500XL power supply"]}
For all stages, set the current limit to 10 mA for 12 gels, . #####%%%%% output "the current limit" are not used
{"action": "set", "output": "the current limit", "volume": ["12 gels"]}