Place tubes in a vacuum desiccator, evacuate overnight in the dark. #####%%%%% output "the dark" are used in line6
{"action": "place", "output": "", "container": ["tubes"], "device": ["vacuum desiccator"], "time": ["overnight"]}
Store lipid dry films in a vacuum desiccator at room temperature, . #####%%%%% output "mineral oil" are used in line6
{"action": "store", "output": "mineral oil", "reagent": ["lipid dry films"], "device": ["vacuum desiccator"], "temperature": ["room temperature"]}
Mix mineral oil by gentle inversion before use.
{"action": "mix", "output": "", "reagent": ["mineral oil"]}
Collect a lipid dry film sample from the desiccator. #####%%%%% output "a lipid dry film sample" are used in line14
{"action": "collect", "output": "a lipid dry film sample", "container": ["the desiccator"]}
Dissolve the lipid dry film by vortexing for 10 s. 10. #####%%%%% output "the lipid-oil mixture" are used in line14
{"action": "dissolve", "output": "the lipid-oil mixture", "reagent": ["the lipid dry film"], "time": ["10 s"]}
If undissolved, repeat steps 8-9.
{"action": "repeat", "output": ""}
Mix the lipid-oil mixture by vortexing for 10 s. 13.
{"action": "mix", "output": "", "reagent": ["the lipid-oil mixture"], "time": ["10 s"]}
Store the lipid-oil mixture at room temperature in the dark, use within 1 week.
{"action": "store", "output": "", "container": ["the lipid-oil mixture"], "temperature": ["room temperature"]}
Prepare a flow chamber using double-sided adhesive tape . #####%%%%% output "lipid-oil mixture" are used in line20
{"action": "prepare", "output": "lipid-oil mixture", "container": ["a flow chamber"]}
Mix lipid-oil mixture by vortexing immediately before use. #####%%%%% output "Valap" are used in line24
{"action": "mix", "output": "Valap", "reagent": ["lipid-oil mixture", "lipid-oil mixture"], "time": [""]}
Perfuse the emulsion into the flow chamber, .
{"action": "perfuse", "output": "", "reagent": ["emulsion"]}
seal with Valap
{"action": "seal", "output": "", "reagent": ["Valap"]}
Observe droplets using an optical microscope.
{"action": "observe", "output": "", "device": ["an optical microscope"]}